SP600125 Attenuates Nicotine-Related Aortic Aneurysm Formation by Inhibiting Matrix Metalloproteinase Production and CC Chemokine-Mediated Macrophage Migration

Figure 7

Transwell assay of macrophage migration. Representative pictures are shown. (a, b) Macrophages that had migrated to the lower chamber were counted after DAPI staining. (c, d) Bar graphs representing quantification of macrophage migration. Nicotine at 0.5 ng/mL or 5 ng/mL induced the strongest macrophage migration, while lower migration was observed following treatment at 50 ng/mL and a significant inhibitory effect was noted at a 500-ng/mL concentration. SP600125 inhibited the migration of macrophages. and versus control; and versus the group treated with 5 ng/mL nicotine.

(a)

(b)

(c)

(d)