Targeting Endothelial Adhesion Molecule Transcription for Treatment of Inflammatory Disease: A Proof-of-Concept Study

Table 1

Primer pairs and product sizes for gene transcripts studied in human retinal endothelial cells. References are provided for primer sequences sourced from the literature. For primer pairs designed in-house, products were confirmed by sequencing.

Gene transcript	Primer pair	Product size (bp)
NF-κB1 [19]	Forward: 5′-CCCAGTGAAGACCACCTCTC-3′	132
	Reverse: 5′-CTGAGTTTGCGGAAGGATGT-3′
ICAM-1 [20]	Forward: 5′-TAAGCCAAGAGGAAGGAGCA-3′	282
	Reverse: 5′-CATATCATCAAGGGTTGGGG-3′
ICAM-1	Forward: 5′-GGCCTCAGTCAGTGTGA-3′	218
	Reverse: 5′-AACCCCATTCAGCGTCA-3′
18S rRNA	Forward: 5′-GTAACCCGTTGAACCCCATT-3′	151
	Reverse: 5′-CCATCCAATCGGTAGTAGCG-3′
Cyclophilin A	Forward: 5′-GACCTCTGGAGAGAAAGGATTT-3′	355
	Reverse: 5′-GGTGATCTTCTTGCTGGTCTT-3′

NF-κB1 = nuclear factor κ-light-chain-enhancer of activated B-cells 1; ICAM-1 = intercellular adhesion molecule 1; 18S rRNA = 18S ribosomal RNA.