Targeting Endothelial Adhesion Molecule Transcription for Treatment of Inflammatory Disease: A Proof-of-Concept Study

Figure 1

Graphs showing relative ICAM-1 transcript expression in human retinal endothelial cell monolayers, following stimulation with TNF-α for 60 minutes or 24 hours, as determined by qRT-PCR using two ICAM-1 primer pairs. Reference gene was 18S rRNA. The same result was obtained when data were normalized to cyclophilin A reference gene. (a) shows the result obtained using ICAM-1 primer pair listed in Table 1 that yields 282 bp product. (b) shows similar result obtained using ICAM-1 primer pair listed in Table 1 that yields 218 bp product. Bars represent mean relative expression, with error bars showing standard error of the mean. monolayers/condition. Data were analyzed by two-tailed Student’s -test. Human retinal endothelial RNA samples used for this experiment were used for PCR array profiling study presented in Table 2.

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(b)