Anti-Inflammatory Effects of Hyperbaric Oxygenation during DSS-Induced Colitis in BALB/c Mice Include Changes in Gene Expression of HIF-1α, Proinflammatory Cytokines, and Antioxidative Enzymes

Figure 5

Relative mRNA expression of IL-1β, IL-2, and IL-6 in the colon, MLN, and spleen (a) and their correlation to HIF-1α gene expression in colonic tissue (b). Relative mRNA expression was measured by real-time PCR; all measured genes were normalized to expression of HPRT1 gene. BALB/c mice at the age of 10–12 weeks were randomly assigned into 4 groups ( min. 5/group/experiment): CTRL: control mice, CTRL + HBO₂: control mice undergoing HBO₂ (60 min/2.4 ATM, 2x/day, days 1–8), DSS: mice receiving dextran sodium sulphate (DSS, 5% w/v, days 1–7), and DSS + HBO₂: DSS treated mice undergoing HBO₂. Data are presented as mean ± s.e.m.; ^∗statistically different from CTRL, ; ^#statistically different from DSS, ; ^†statistically different from CTRL + HBO₂, .

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(b)