Expression of Interferon Effector Gene SART1 Correlates with Interferon Treatment Response against Hepatitis B Infection

Figure 2

Silencing SART1 antagonizes the antiviral activity of IFN-α. (a–c) HepG2 cells were cotransfected with HBV-1.3 and Si-SART1 for 24 h. The cells were then treated with 1000 IU/mL IFN-α for 24 h. 48 h after transfection, the supernatants were collected and assayed for quantification of HBV DNA by real-time PCR, HBeAg, and HBsAg by ELISA. An siRNA-negative control was used as a control. (d) HepG2 cells were transfected with si-SART1. 48 h after transfection, the efficiency of si-SART1 was detected by qRT-PCR and western blot. Means ± SD from three independent experiments performed in triplicate are shown. Student’s -test was used to determine significance of differences between two groups. , and .

(a)

(b)

(c)

(d)