CETP Lowers TLR4 Expression Which Attenuates the Inflammatory Response Induced by LPS and Polymicrobial Sepsis

Figure 7

CETP reduces LPS-stimulated transcription factor NF-κB activation. Peritoneal macrophages obtained from WT mice were stimulated for 24 h with LPS-Alexa Fluor 488 (1 µg/mL) in the absence or presence of CETP (0.1, 0.5, and 1.0 µg/mL). (a) Representative FACS dot plots gated on F4/80⁺ cells (macrophages only) and the percentage of NF-κB⁺cells are shown. (b) Representative flow cytometry histograms of NF-κB comparing macrophages (F4/80⁺) from WT and respective CETP groups stimulated with LPS. (c) Graph presented as mean ± SD of MFI (median fluorescence intensity) analyzed using FACSDiva software ( = 4) representative of three independent experiments, by one-way ANOVA and posttest (Newman-Keuls Multiple Comparison Test; and ).

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