Sphingolipids as Regulators of the Phagocytic Response to Fungal Infections

Figure 1

Neutrophils are important to control cryptococcosis in mice. Four six-week-old mice (CBA/J or SJL/J model) were treated intraperitoneally every other day with 300 μg of Rb6-8C5 monoclonal antibody (mAb, gray arrows) directed against neutrophils. After 2 days from the first dose (day 0), mice were infected intranasally with a lethal dose of C. neoformans cells () (black arrow). As controls, mice were treated with LTF-2 mAB (an IgG2 isotype for Rb6-8C5). Before mAb treatment and C. neoformans challenge, and during infection, blood was collected for neutrophil count (red arrows).