Increased Expression of Chitinase 3-Like 1 in Aorta of Patients with Atherosclerosis and Suppression of Atherosclerosis in Apolipoprotein E-Knockout Mice by Chitinase 3-Like 1 Gene Silencing
Figure 4
(a)–(d) Real-time RT-PCR quantification of inflammatory cytokines mRNA expression in mice carotid plaques in control group and silenced group. The silenced group showed lower mRNA expression levels of TNF-α, MCP-1, IL-8, and MMP-9, compared with the control group, and inhibition of CHI3L1 reduced the inductions of proinflammatory cytokines. (e)-(F) Western blot analysis and quantification of ERK1/2, phospho-ERK1/2, AKT, and phospho-AKT protein expression in control group and silenced group. The protein expression levels of phospho-ERK1/2 and phospho-AKT were decreased in silenced group compared with that in control group. versus control group.
