Regulatory T Cells Protect Fine Particulate Matter-Induced Inflammatory Responses in Human Umbilical Vein Endothelial Cells

Figure 8

Tregs downregulate NF-κB activation in HUVECs impaired by PM. The electrophoretic mobility shift assay (EMSA) was conducted with nuclear proteins isolated from different HUVEC cultures to detect the NF-κB activity. (a) Representative EMSA results. (b) The DNA-binding activity of NF-κB in different groups determined by the relative measurement method. Data are expressed as means ± SEM. ∗ indicates no T, CD4⁺CD25⁻, or CD4⁺CD25⁺ versus control; # indicates no T or CD4⁺CD25⁻ versus CD4⁺CD25⁺. ^∗∗𝑃<0.01, ^#𝑃<0.05, and ^##𝑃<0.01. Experiments were repeated 4 times.

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