Regulatory T Cells Protect Fine Particulate Matter-Induced Inflammatory Responses in Human Umbilical Vein Endothelial Cells

Figure 1

Isolation and identification of CD4⁺CD25⁺ T cells. (a) The purity of CD4⁺CD25⁺ T cells isolated from peripheral blood mononuclear cells (PBMCs) of healthy volunteers was examined by flow cytometry. (b) The percentage of the foxp3⁺ population among the sorted CD4⁺CD25⁺ T cells. (c) Proliferation was evaluated by thymidine incorporation. The relative effect of CD4⁺CD25⁺ T cells was expressed as percentage inhibition of CD4⁺CD25⁻ T cells. Experiments were repeated 3 times.

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