The Influence of Ouabain on Human Dendritic Cells Maturation

Figure 5

CD86 and CD80 expression on dendritic cells during activation. After the differentiation period (5 days), cells were incubated for further 48 h with or without TNF-α (50 ng/mL), in the presence or absence of 100 nM Oua. CD86 and CD80 expression was analyzed by flow cytometry. Data are expressed as (a, c) the percentage of positive cells, or (b, d) MFI (mean fluorescence intensity), and lines denote the means of five independent experiments. ∗∗ and ∗∗∗ are significantly different from the respective controls (^∗∗; ^∗∗∗).

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