The Effects of Florfenicol on the Values of Serum Tumor Necrosis Factor-𝜶 and Other Biochemical Markers in Lipopolysaccharide-Induced Endotoxemia in Brown Trout

Table 1

The effect of florfenicol (F) on sTNF-𝛼 and serum biochemical indices in endotoxemic fish (mean ± SE).

	Groups	0 hour	1.5 hour	3 hours	6 hours	10 hours	24 hours
TNF-𝛼 (pg/mL)	LPS	728±188^b	2064±244^a	2094±474^a	1022±412^ab	1642±606^ab	736±232^b
	LPS + F	728±188^b	2313±321^a	810±425^b	1254±440^ab	1101±480^ab	1876±536^ab
	F	728±188^a	1681±545^a	1462±439^a	1196±264^a	976±431^a	1208±228^a
ALP (U/L)	LPS	90.0±11.4^b	87.0±16.8^b	87.0±11.1^b	160±15.0^a	94.0±7.77^b	123±11.7^b
	LPS + F	90.0±11.4^a	93.0±13.0^a	90.0±5.46^a	128±18.7^a	116±17.3^a	103±13.8^a
	F	90.0±11.4^ab	82.0±17.0^b	109±23.2^ab	132±12.9^a	107±13.5^ab	106±3.57^ab
ALT (U/L)	LPS	4.50±0.43^b	4.83±0.95^b	4.67±0.49^b	18.7±4.70^a	8.83±2.18^b	4.83±0.31^b
	LPS + F	4.50±0.43^d	7.17±1.11^cd	10.3±0.72^bcd	14.8±2.24^ab	18.2±4.37^a	12.7±2.01^abc
	F	4.50±0.43^c	9.67±1.15^ab	7.67±0.92^bc	12.8±2.20^a	12.2±0.95^a	11.5±1.09^a
AST (U/L)	LPS	223±27.3^c	220±27.2^c	242±16.0^bc	477±51.6^a	323±25.2^b	325±18.0^b
	LPS + F	223±27.3^c	281±41.5^c	343±33.9^bc	469±43.7^a	405±54.8^ab	466±30.7^a
	F	223±27.3^d	344±44.5^bc	285±12.3^cd	499±48.5^a	440±34.5^ab	523±41.3^a
Total protein (g/dL)	LPS	3.80±0.48^b	5.40±0.51^a	3.70±0.31^b	3.60±0.30^b	3.40±0.36^b	3.30±0.08^b
	LPS + F	3.80±0.48^a	3.40±0.38^a	3.40±0.14^a	3.10±0.17^a	3.70±0.26^a	3.30±0.15^a
	F	3.80±0.48^a	3.00±0.21^ab	3.20±0.13^ab	2.70±0.18^b	3.30±0.25^ab	3.30±0.13^ab
Albumin (g/dL)	LPS	1.90±0.12^a	1.90±0.08^a	1.50±0.03^b	1.90±0.14^a	1.70±0.10^ab	1.80±0.05^a
	LPS + F	1.90±0.12^a	2.00±0.24^a	1.70±0.03^a	1.80±0.02^a	2.00±0.09^a	1.80±0.05^a
	F	1.90±0.12^a	1.70±0.14^a	1.70±0.07^a	1.90±0.22^a	1.90±0.07^a	1.80±0.09^a
Creatinine (mg/dL)	LPS	0.26±0.03^cd	0.93±0.12^a	0.38±0.02^bc	0.51±0.03^b	0.35±0.03^c	0.15±0.01^d
	LPS + F	0.26±0.03^bc	0.22±0.01^cd	0.20±0.02^cd	0.38±0.03^a	0.33±0.04^ab	0.17±0.01^d
	F	0.26±0.03^a	0.21±0.02^ab	0.20±0.01^ab	0.16±0.01^b	0.21±0.02^ab	0.22±0.02^ab
BUN (mg/dL)	LPS	4.50±0.67^b	6.67±0.99^a	3.83±0.40^b	6.83±0.60^a	4.33±0.80^b	4.33±0.33^b
	LPS + F	4.50±0.67^a	5.83±1.72^a	3.83±0.48^a	6.33±0.76^a	3.50±0.56^a	3.83±0.95^a
	F	4.50±0.67^a	5.17±0.70^a	4.67±0.42^a	5.83±0.91^a	6.00±0.68^a	6.00±0.58^a
HDL (mg/dL)	LPS	123±3.61^bc	101±7.34^c	108±8.00^c	151±9.33^a	132±9.34^ab	147±5.69^a
	LPS + F	123±3.61^bc	109±10.7^c	140±5.49^ab	133±5.43^ab	152±6.41^a	131±7.70^ab
	F	123±3.61^b	131±11.1^b	134±3.46^b	193±18.8^a	151±2.31^b	150±5.71^b
LDL (mg/dL)	LPS	35.0±1.67^bc	39.0±4.95^bc	32.0±2.47^c	46.0±4.62^ab	39.0±2.18^bc	51.0±4.30^a
	LPS + F	35.0±1.67^a	34.0±3.20^a	46.0±5.16^a	48.0±6.21^a	47.0±3.94^a	39.0±3.75^a
	F	35.0±1.67^bc	33.0±5.00^c	35.0±3.77^bc	46.0±1.38^a	42.0±3.23^abc	44.0±2.35^ab
TG (mg/dL)	LPS	377±55.3^b	562±95.1^a	243±19.7^b	340±59.9^b	245±23.1^b	239±23.1^b
	LPS + F	377±55.3^a	369±66.1^a	241±33.6^a	249±20.5^a	291±33.9^a	257±21.7^a
	F	377±55.3^a	194±25.6^c	284±34.2^abc	340±42.1^ab	185±16.1^c	260±26.2^bc

LPS: lipopolysaccharide, F: florfenicol, TNF-𝛼: tumor necrosis factor alpha, ALP: alkaline phosphatase, ALT: alanine aminotransferase, AST: aspartate aminotransferase, BUN: blood urea nitrogen, HDL: high density lipoproteins, LDL: low density lipoproteins, TG: triglycerides, and ^{a, b, c, d}different letters on the same line refer to statistical significance (𝑃<0.05).