Interaction of Apoptotic Cells with Macrophages Upregulates COX-2/PGE2 and HGF Expression via a Positive Feedback Loop
Figure 7
HGF receptor antagonist suppresses COX-2 expression and PGE2 production in peritoneal macrophages in response to apoptotic cells. Murine peritoneal macrophages were pretreated with PHA-665752 (1 or 10 μM) for 1 h and then stimulated with apoptotic Jurkat cells (ApoJ). (a) At 2 h after in vitro exposure to ApoJ, COX-2 mRNA levels were analyzed by semiquantitative RT-PCR. At 24 h after in vitro exposure to ApoJ, total cell lysates were immunoblotted for COX-2 (b) and cell supernatants were measured for PGE2 by EIA (c). Values represent mean ± SEM of three or more separate experiments; .
