Interaction of Apoptotic Cells with Macrophages Upregulates COX-2/PGE₂ and HGF Expression via a Positive Feedback Loop

Figure 2

Apoptotic cells induce PGE₂ and 15d-PGJ₂ production by RAW 264.7 cells. (a, c) RAW 264.7 cells were stimulated by apoptotic (ApoJ), viable (ViaJ) Jurkat T cells, or lipopolysaccharide (LPS) for the times indicated. (b, d) After 1 h pretreatment with 1–50 μM of NS-398 or 10 μM of indomethacin (Indo), RAW cells were stimulated with ApoJ for 2 h or 24 h. PGE₂ or 15d-PGJ₂ levels in the conditioned media were measured by EIA. Values represent mean ± SEM of three or more separate experiments, ^∗𝑃<0.05, compared with control or ApoJ versus ApoJ + NS or Indo.

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