The Protective Effect of Lidocaine on Septic Rats via the Inhibition of High Mobility Group Box 1 Expression and NF-𝜿B Activation

Figure 5

Lidocaine treatment inhibits translocation and activity of NF-κB in organs of CLP-induced septic rats. Rats were treated with NS or lidocaine 10 h after CLP. Liver, kidneys, lungs, and ileum were collected 24 h after CLP (a, b, c, and d). The nuclear fraction was collected and NF-κB levels in the nuclear fraction were measured using the Active Motif NF-κB family kit. Data are presented as mean ± SD (𝑛=5). ^a𝑃<0.001 versus Sham; ^b𝑃<0.001 versus NS; ^c𝑃<0.001 versus Lido3 mg/kg; ^d𝑃<0.001 versus Lido6 mg/kg. (e) Immunohistochemical staining of nuclear factor-κB p65 in liver, kidneys, lungs, and ileum from rats subjected to Sham and to CLP treated with NS and rats subjected to CLP treated with lidocaine 9 mg/kg at 24 h after CLP. Original magnification: ×400; NS: normal saline; Lido: lidocaine.

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