Research Article
Plasmacytoid Dendritic Cell Response to CpG ODN Correlates with CXCL16 Expression and Is Inhibited by ox-LDL
Table 1
Altering CXCL16 expression influences “D” ODN induced cytokine production.
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PBMC from 5-6 different donors were treated with 50 M of GM6001 or 25 g/mL of o-sialoglycoprotein endopeptidase (from Pasteurella haemolytica) for 30 min at 37°C. The cells were then stimulated in vitro with 3 M “D” ODN for 24 h, and the production of IFN determined by ELISA. The fraction of CD123/BDCA-2 double positive pDC expressing CXCL16 was determined before and after Rx. Treatment-induced changes were calculated for each donor independently and then averaged. . |