Plasmacytoid Dendritic Cell Response to CpG ODN Correlates with CXCL16 Expression and Is Inhibited by ox-LDL

Figure 1

Metalloproteinase inhibitor GM-6001 enhances cytokine production induced by D-ODN.   (a)  PBMC (4 × 10⁶/mL) were preincubated in the absence or presence of GM6001 (50 μM) for 30 min, fixed, and stained for CXCL16 expression. Cells treated as in (a)  were washed and then stimulated with 3 μM D-ODN (b), 1 μM of C ODN (c), or 1 μM of K-ODN (d) for 24 h. Cytokine production (IL-6 for K-ODN and IFNα for D and C ODN) was assessed from culture supernatants using ELISA. Response of 6 individual donors is shown.

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