Lipoxin A₄ Preconditioning and Postconditioning Protect Myocardial Ischemia/Reperfusion Injury in Rats

Figure 6

Protein levels of 𝛼₁, 𝛼₂, and 𝛼₃ and 𝛽₁ subunits of Na⁺-K⁺-ATPase of cardiac tissues from rats of different groups at T₂. Samples were collected right after I/R procedure was over and western blot was performed as described in the section of Materials and Methods, and 𝛽-Actin was used as a loading control. Representative blots of three independent experiments were shown. (a) Western blotting: (A) 𝛼₁ subunit of Na⁺-K⁺-ATPase; (B) 𝛼₂ subunit; (C) 𝛼₃ subunit; (D) 𝛽₁ subunit. (b) Quantitative data of western blot: (A) 𝛼₁ subunit of Na⁺-K⁺-ATPase; (B) 𝛼₂ subunit; (C) 𝛼₃ subunit; (D) 𝛽₁ subunit. *𝑃<0.05 for comparisons of I/R₁ and LX₁ groups with C₁ group, 𝑃<0.05 for comparisons of I/R₂ and LX₂ with C₂ group; ^#𝑃<0.05 for comparisons of LX₁ group with I/R₁ group, 𝑃<0.05 for comparisons of LX₂ group with I/R₂ group; ^Δ𝑃<0.05 for comparisons of LX₂ group with LX₁ group.

(a)

(b)