oxLDL Downregulates the Dendritic Cell Homing Factors CCR7 and CCL21
Figure 4
mRNA expression of CCL21/19 and protein expression of CCL21 on HMECs-1 after stimulation with oxLDL. (a) Fold increase in mRNA expression levels of CCL21/19 in human microvascular endothelial cells after 48 h of stimulation with oxLDL (10 μg/mL) analyzed by real-time PCR. *, () versus unstimulated controls. (b) Corresponding protein expression of CCL21 measured by immunofluorescence analysis. The green staining represents CCL21 positive structures (α-CCL21/6Ckine-Alexa488), red represents the plasma membrane (Vybrand DiD). Scale bar 10 μm. (c) The fluorescence intensity was quantified showing a significant change for oxLDL. *, () versus unstimulated control.