Mediators of Inflammation / 2011 / Article / Fig 5

Research Article

Dual Specificity Phosphatase 1 Regulates Human Inducible Nitric Oxide Synthase Expression by p38 MAP Kinase

Figure 5

Expression of DUSP1 in response to cytokine mixture or LPS in A549 and J774 cells. Cells were stimulated with (a) cytokines (CM: TNF, IFNγ, and IL-1β; 10 ng/mL each) or (b) LPS (10 ng/mL) for the time indicated, and DUSP1 mRNA and protein expression was determined by quantitative real-time RT-PCR and Western blot, respectively. DUSP1 mRNA expression was normalized against GAPDH mRNA. In Western blots, chemiluminescent signal was quantified and DUSP1 protein expression was normalized against actin. The gels are representatives of six separate experiments with similar results. DUSP1 protein and mRNA levels are expressed in arbitrary units, DUSP1 expression in unstimulated cells (0 h) is set as 1, and the other values are related to that (mean ± S.E.M.; ). One-way ANOVA with Dunnett’s posttest was performed, and statistical significance is indicated with and compared with unstimulated cells.

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