Upregulation of Salmonella-Induced IL-6 Production in Caco-2 Cells by PJ-34, PARP-1 Inhibitor: Involvement of PI3K, p38 MAPK, ERK, JNK, and NF-B
Figure 4
Effect of PJ-34 on Salmonella-activated intracellular signals. Caco-2 cells were left untreated, or treated with 40M PJ-34, and then infected with wild-type S. Typhimurium strain SL1344 for the times indicated. Activation of the AKT pathway was analyzed in cell fraction (membrane part) by immunoblotting with antibodies to phosphorylated (p) AKT and total AKT. E-cadherin works as a normalization of membranous protein. The results shown are representative of 3 separate experiments. The activation and recruitment of Akt were not significantly suppressed by PJ-34.
