Tuberculosis Caused by Mycobacterium bovis in a Sheep Flock Colocated with a Tuberculous Dairy Cattle Herd in Central Ethiopia

Figure 4

Spoligotype patterns of M. bovis isolates from cattle and sheep. The first two samples (BO1 and BO2) were isolates obtained from bovine species (cows) in contact with sheep, while the rest (OV1, OV2, OV3, and OV4) were obtained from the four sheep that were positive to CIDT test and postmortem examination. Positive controls: H37Rv=M. tuberculosis H37Rv; M. bovis 2122/97, Negative control: Qiagen water (QH₂O). Both positive control strains yielded standard patterns of spacer arrangements. This is signified by absence of spacers 20-21 and 33-36 and presence of spacers 39-43 for M. tuberculosis H37Rv; and absence of spacers 3, 9, 16 and 39-43 for M. bovis BCG.