Isolation, Purification, and Characterization of Homogenous Novel Bioactive Protein from Datura stramonium Stem Exhibited Larvicidal Activity against Anopheles stephensi

Table 3

The LC₅₀ and LC₉₀ values of crude and purified plant proteins against third instar larvae of An. stephensi.

Mosquito species	Protein extract	LC50 (mg/ml)	LC90 (mg/ml)	Regression equation	R^2	95% confidence intervals (LCL–UCL)
An. stephensi	Crude protein	0.489	5.43	Y = 0.0221x + 4.259	0.896	5.08–5.68
	Purified protein	0.025	0.04	Y = 0.0391x + 3.357	0.9534	1.73–6.28

R = coefficient of regression equations; LC₅₀ = lethal concentration that kills 50% of larvae; LC₉₀ = lethal concentration that kills 90% of larvae.