Expression, Purification, and Evaluation of Diagnostic Potential and Immunogenicity of a Recombinant NS3 Protein from All Serotypes of Dengue Virus
Figure 3
Seroprevalence and serotyping of dengue virus. (a) Geographical distribution of cases in the municipality of Tezonapa; the circles on the map indicate the villages, with their size proportional to the number of samples as indicated on the map, white areas represent IgG-positive samples, dark gray areas represent IgM-positive samples, light gray areas represent NS1-positive samples, and black areas represent NS3-positive samples. (b) Identification of positive samples for IgM and IgG by ELISA (Panbio). (c) NS1 antigen detection (Bio-Rad) and identification of NS3-positive samples by ELISA (in-house detection system). (d) Serotyping of dengue virus by RT-PCR, lanes 1–5 representative samples from rural villages; heat arrow indicates PCR product by DEN 1 and DEN 2.
