An Assessment of the Genotoxicity and Subchronic Toxicity of a Supercritical Fluid Extract of the Aerial Parts of Hemp
Table 7
(a) Summary of body weights in the 90-day repeated oral toxicity study
Group (mg/kg bw/day)
Body weight (g) on days
0
3
7
10
14
17
21
24
28
35
42
49
56
63
70
77
84
89
Males
Control
170.9 ± 10.1
190.3 ± 10.2
214.7 ± 11.0
232.9 ±10.6
252.1 ± 12.2
267.1 ± 12.8
281.5 ± 13.8
292.7 ± 14.9
305.0 ± 15.5
325.7 ± 16.8
338.7 ± 17.5
351.9 ± 20.1
360.7 ± 20.8
369.5 ± 21.3
379.7 ± 22.7
385.8 ± 23.8
397.1 ± 26.0
397.3 ± 25.4
100
172.0 ±8.4
191.3 ± 10.9
214.9 ± 13.3
231.8 ± 16.9
251.3 ± 20.8
266.5 ± 23.2
280.7 ± 26.2
293.0 ± 29.1
304.6 ± 32.3
325.1 ± 36.0
337.5 ± 37.7
349.0 ± 39.4
354.8 ± 39.3
361.8 ± 41.5
370.2 ± 42.9
371.8 ± 42.7
382.5 ± 43.1
386.1 ± 45.1
360
171.1 ± 8.6
180.7 ± 7.6
199.4 ± 8.9∗∗
215.1 ± 9.2∗∗
230.9 ± 9.3∗∗
244.8 ± 8.9∗∗
257.6 ± 9.8∗∗
268.3 ± 10.9∗∗
278.8 ± 11.3∗∗
296.3 ± 12.8∗∗
308.7 ± 14.8∗∗
318.3 ± 15.5∗∗
323.2 ± 17.6∗∗
332.0 ± 19.4∗∗
341.5 ± 19.8∗∗
344.5 ± 20.5∗∗
356.7 ± 22.7∗∗
358.4 ± 19.5∗∗
720
171.3 ± 7.1
175.2 ± 6.5∗∗
193.1 ± 6.2∗∗
205.5 ± 7.5∗∗
222.3 ±8.7∗∗
233.3 ± 9.6∗∗
247.0 ± 11.7∗∗
256.4 ± 12.5∗∗
266.9 ± 12.7∗∗
281.9 ± 12.3∗∗
294.8 ± 12.6∗∗
303.5 ± 12.4∗∗
311.3 ± 12.8∗∗
317.6 ± 14.5∗∗
326.0 ± 14.5∗∗
327.1 ± 14.8∗∗
339.7 ± 16.2∗∗
341.0 ± 17.9∗∗
Females
Control
124.3 ± 10.4
135.1 ± 10.8
147.1 ± 11.1
155.7 ± 10.1
164.3 ± 10.3
173.1 ± 10.0
179.8 ± 10.8
186.9 ± 10.8
193.3 ± 11.5
205.3 ± 12.7
213.5 ± 13.0
221.1 ± 14.6
224.9 ± 12.7
231.2 ± 14.6
236.0 ± 15.2
236.5 ± 15.9
245.1 ± 15.6
244.5 ± 17.0
100
125.7 ± 10.6
134.8 ± 10.0
146.0 ± 10.2
153.7 ± 11.4
162.8 ± 10.9
171.2 ± 9.9
178.5 ± 13.0
187.0 ± 13.0
193.6 ± 12.9
204.3 ± 13.7
210.3 ± 13.0
218.1 ± 14.9
221.8 ± 13.5
226.7 ± 14.6
232.4 ± 14.7
230.9 ± 16.0
241.8 ± 16.4
242.1 ± 17.1
360
122.8 ± 10.8
128.1 ± 10.7
138.9 ± 10.7
146.6 ± 10.8
156.5 ± 10.7
165.8 ± 11.5
173.3 ± 12.0
180.7 ± 11.9
186.4 ± 12.5
200.2 ± 13.1
206.8 ± 12.3
211.5 ± 13.5
216.3 ± 14.1
221.2 ± 13.3
224.3 ± 14.2
225.0 ± 13.2
231.7 ± 12.7
233.0 ± 11.0
720
123.7 ± 10.0
124.5 ± 8.6
132.9 ± 10.5∗∗
141.1 ± 10.3∗∗
151.3 ± 11.8∗∗
160.1 ± 10.4∗∗
169.1 ± 10.4
175.5 ± 10.1
182.5 ± 9.6
191.0 ± 10.6∗∗
198.4 ± 10.4∗∗
203.3 ± 10.8∗∗
206.4 ± 11.3∗∗
211.5 ± 10.5∗∗
214.2 ± 11.1∗∗
212.6 ± 11.8∗∗
219.1 ± 10.8∗∗
220.9 ± 11.1∗∗
= 15 in control and 720 mg/kg bw/day groups; in all other groups = 10. , ∗∗p < 0.01, statistical significances were determined with Duncan's multiple range test or with Mann–Whitney test versus control.
(b) Summary of body weight in the recovery period
Group (mg/kg bw/day)
Body weight (g) on days
96
103
110
117
Males
Control
422.0 ± 14.7
431.4 ± 17.6
440.6 ± 16.8
445.6 ± 16.4
720
344.6 ± 11.7∗∗
360.4 ± 14.0∗∗
373.2 ± 17.5∗∗
384.6 ± 22.1∗∗
Females
Control
244.6 ± 9.1
247.6 ± 10.4
252.6 ± 10.0
254.8 ± 10.1
720
215.6 ± 9.8∗∗
218.0 ± 10.6∗∗
220.4 ± 14.9∗∗
226.8 ± 9.5∗∗
= 5 for all groups. p < 0.05, ∗∗p < 0.01, statistical significances were determined with Duncan's multiple range test or with Mann–Whitney test versus control.