An Assessment of the Genotoxicity and Subchronic Toxicity of a Supercritical Fluid Extract of the Aerial Parts of Hemp
Table 2
Summary table of the results of the confirmatory mutation test.
Confirmatory mutation test (preincubation test)
Concentrations (g/plate)
Salmonella typhimurium tester strains
Escherichia coli
TA 98
TA 100
TA 1535
TA 1537
WP2uvrA
-S9
+S9
-S9
+S9
-S9
+S9
-S9
+S9
-S9
+S9
Mean values of revertants per plate and mutation rate (MR)
Mean
MR
Mean
MR
Mean
MR
Mean
MR
Mean
MR
Mean
MR
Mean
MR
Mean
MR
Mean
MR
Mean
MR
Untreated control
14.7
0.96
22.7
1.33
102.0
1.02
126.3
1.10
8.7
0.93
8.0
0.83
9.3
1.27
9.0
0.84
13.3
0.77
19.3
1.16
DMSO control
15.3
1.00
17.0
1.00
100.0
1.00
114.3
1.00
9.3
1.00
9.7
1.00
7.3
1.00
10.7
1.00
17.3
1.00
16.7
1.00
Ultrapure water control
–
–
–
–
86.7
1.00
–
–
13.0
1.00
–
–
–
–
–
–
17.7
1.00
–
–
5000
5.0
0.33
16.7
0.98
44.3
0.44
61.3
0.54
0.3
0.04
9.0
0.93
0.7
0.09
4.7
0.44
12.7
0.73
16.3
0.98
1600
9.0
0.59
23.3
1.37
46.7
0.47
71.3
0.62
2.7
0.29
8.3
0.86
2.0
0.27
8.7
0.81
16.3
0.94
17.7
1.06
500
8.3
0.54
18.0
1.06
45.7
0.46
69.7
0.61
6.0
0.64
9.3
0.97
0.7
0.09
8.3
0.78
14.3
0.83
15.3
0.92
160
12.3
0.80
26.7
1.57
80.3
0.80
91.0
0.80
8.3
0.89
10.0
1.03
4.3
0.59
9.3
0.88
10.7
0.62
20.3
1.22
50
12.3
0.80
26.3
1.55
94.7
0.95
100.7
0.88
10.0
1.07
10.0
1.03
7.7
1.05
10.3
0.97
16.0
0.92
18.0
1.08
16
14.0
0.91
29.0
1.71
94.7
0.95
101.0
0.88
9.3
1.00
8.7
0.90
7.7
1.05
11.7
1.09
12.3
0.71
19.3
1.16
5
14.3
0.93
23.0
1.35
94.7
0.95
115.7
1.01
10.3
1.11
13.0
1.34
7.3
1.00
11.3
1.06
16.0
0.92
18.3
1.10
NPD (4)
310.0
20.22
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
SAZ (2)
–
–
–
–
1389.3
16.03
–
–
3104.0
238.77
–
–
–
–
–
–
–
–
–
–
9AA (50)
–
–
–
–
–
–
–
–
–
–
–
–
586.0
79.91
–
–
–
–
–
–
MMS (2 μL)
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
898.7
50.87
–
–
2AA (2)
–
–
989.3
58.20
–
–
1752.0
15.32
–
–
113.3
11.72
–
–
133.7
12.53
–
–
–
–
2AA (50)
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
181.3
10.88
DMSO was applied as vehicle of the test article and positive control substances: NPD, 9AA, and 2AA; and the ultrapure water was applied as vehicle for the SAZ and MMS. The mutation rate of the test item and the untreated control is given referring to the DMSO.
