Research Article

An Assessment of the Genotoxicity and Subchronic Toxicity of a Supercritical Fluid Extract of the Aerial Parts of Hemp

Table 13

Summary of relative organ weights (organ weight relative to body weight) (%) in the 90-day repeated oral toxicity study.

Group (mg/kg bw/day)BrainLiverKidneysHeartThymusSpleenTestes/uterusEpididymides/ovariesAdrenalsThyroid

Male (main groups, = 10 each)
Control0.539 ± 0.0282.515 ± 0.0980.554 ± 0.0360.284 ± 0.0180.103 ± 0.0210.165 ± 0.0260.902 ± 0.0510.441 ± 0.0520.018 ± 0.0020.006 ± 0.002
1000.545 ± 0.0542.595 ± 0.0810.570 ± 0.0400.281 ± 0.0340.094 ± 0.0120.157 ± 0.0110.933 ± 0.0540.426 ± 0.0380.019 ± 0.0030.005 ± 0.002
3600.581 ± 0.0433.196 ± 0.147∗∗0.591 ± 0.0440.272 ± 0.0190.090 ± 0.0240.158 ± 0.0250.938 ± 0.0860.430 ± 0.0490.023 ± 0.004∗∗0.006 ± 0.001
7200.617 ± 0.055∗∗4.093 ± 0.228∗∗0.631 ± 0.027∗∗0.290 ± 0.0280.088 ± 0.0200.168 ± 0.0211.002 ± 0.080∗∗0.414 ± 0.0260.024 ± 0.004∗∗0.007 ± 0.002
Historical range0.441–0.5991.916–3.1080.466–0.6500.216–0.3110.057–0.1910.058–0.2190.642–1.0110.320–0.5120.012–0.0230.0037–0.0089

Male (recovery group, = 5 each)
Control0.478 ± 0.0152.454 ± 0.0890.522 ± 0.0190.236 ± 0.0150.087 ± 0.0150.158 ± 0.0190.806 ± 0.0140.418 ± 0.0270.017 ± 0.0020.006 ± 0.000
7200.550 ± 0.032∗∗2.392 ± 0.1580.587 ± 0.037∗∗0.265 ± 0.0200.098 ± 0.0210.168 ± 0.0230.941 ± 0.082∗∗0.460 ± 0.0320.017 ± 0.0010.007 ± 0.000∗∗

Female (main groups, = 10 each)
Control0.825 ± 0.0282.693 ± 0.1740.625 ± 0.0400.322 ± 0.0220.128 ± 0.0180.230 ± 0.0340.327 ± 0.0880.0669 ± 0.01100.0348 ± 0.00430.0096 ± 0.0022
1000.805 ± 0.0722.854 ± 0.1330.609 ± 0.0240.324 ± 0.0270.159 ± 0.024∗∗0.196 ± 0.023∗∗0.251 ± 0.0510.0616 ± 0.01620.0357 ± 0.00510.0083 ± 0.0018
3600.853 ± 0.0943.298 ± 0.144∗∗0.598 ± 0.0340.317 ± 0.0330.145 ± 0.0260.191 ± 0.023∗∗0.224 ± 0.094∗∗0.0627 ± 0.01080.0413 ± 0.00540.0091 ± 0.0027
7200.882 ± 0.0554.322 ± 0.281∗∗0.637 ± 0.0380.323 ± 0.0250.141 ± 0.0170.169 ± 0.018∗∗0.215 ± 0.055∗∗0.0653 ± 0.01630.0469 ± 0.0068∗∗0.0109 ± 0.0037
Historical range0.681–0.9432.172–3.2140.530–0.7520.273–0.3960.093–0.2170.140–0.2980.167–0.8520.043–0.0860.026–0.0440.0052–0.0128

Female (recovery group, = 5 each)
Control0.820 ± 0.0232.671 ± 0.1670.633 ± 0.0320.299 ± 0.0120.151 ± 0.0200.196 ± 0.0200.290 ± 0.0450.0562 ± 0.01370.0285 ± 0.00310.0090 ± 0.0004
7200.885 ± 0.0432.700 ± 0.0640.629 ± 0.0140.309 ± 0.0160.152 ± 0.0080.197 ± 0.0230.221 ± 0.0470.0600 ± 0.01360.0345 ± 0.00270.0109 ± 0.0010∗∗

Data represent the mean values and the standard deviation.
and ∗∗P < 0.01, statistical significances were determined with Duncan's multiple range test or with Mann–Whitney test versus control.
: minimum and maximum levels reported as the range of historical control values.