An Assessment of the Genotoxicity and Subchronic Toxicity of a Supercritical Fluid Extract of the Aerial Parts of Hemp
Table 12
Summary of organ weights (g) in the 90-day repeated oral toxicity study.
Group (mg/kg bw/day)
Body weight
Brain
Liver
Kidneys
Heart
Thymus
Spleen
Testes/uterus
Epididymides/ovaries
Adrenals
Thyroid
Male (main groups, = 10 each)
Control
383.3 ± 27.86
2.06 ± 0.14
9.65 ± 0.95
2.12 ± 0.18
1.09 ± 0.09
0.40 ± 0.09
0.64 ± 0.11
3.45 ± 0.20
1.68 ± 0.13
0.068 ± 0.008
0.023 ± 0.006
100
374.1 ± 42.85
2.02 ± 0.09
9.70 ± 1.04
2.13 ± 0.27
1.05 ± 0.14
0.35 ± 0.08
0.59 ± 0.07
3.48 ± 0.29
1.59 ± 0.14
0.072 ± 0.008
0.020 ± 0.006
360
345.7 ± 18.90∗∗
2.01 ± 0.11
11.05 ± 0.79∗∗
2.04 ± 0.16
0.94 ± 0.09∗∗
0.31 ± 0.09
0.55 ± 0.09
3.24 ± 0.27
1.48 ± 0.16∗∗
0.080 ± 0.012
0.022 ± 0.004
720
329.4 ± 18.86∗∗
2.02 ± 0.12
13.50 ± 1.37∗∗
2.08 ± 0.15
0.95 ± 0.08∗∗
0.29 ± 0.07∗∗
0.55 ± 0.07
3.29 ± 0.25
1.36 ± 0.11∗∗
0.080 ± 0.012
0.023 ± 0.005
Historical range♮
344–488
1.81–2.39
7.95–14.14
1.79–2.73
0.97–1.50
0.23–0.84
0.26–0.96
2.58–4.20
1.39–1.93
0.047–0.097
0.015–0.038
Male (recovery group, = 5 each)
Control
434.0 ± 16.08
2.07 ± 0.01
10.65 ± 0.44
2.26 ± 0.08
1.02 ± 0.04
0.38 ± 0.07
0.68 ± 0.08
3.50 ± 0.09
1.81 ± 0.09
0.071 ± 0.007
0.025 ± 0.002
720
373.2 ± 19.25∗∗
2.05 ± 0.06
8.93 ± 0.81∗∗
2.19 ± 0.09
0.99 ± 0.03
0.36 ± 0.07
0.63 ± 0.08
3.51 ± 0.29
1.71 ± 0.08
0.063 ± 0.003
0.026 ± 0.003
Female (main groups, = 10 each)
Control
239.0 ± 18.90
1.97 ± 0.12
6.42 ± 0.40
1.49 ± 0.16
0.77 ± 0.07
0.30 ± 0.04
0.55 ± 0.10
0.79 ± 0.23
0.159 ± 0.025
0.083 ± 0.013
0.023 ± 0.005
100
234.6 ± 15.97
1.88 ± 0.15
6.70 ± 0.56
1.43 ± 0.09
0.76 ± 0.05
0.37 ± 0.05∗∗
0.46 ± 0.06∗∗
0.59 ± 0.13
0.145 ± 0.041
0.084 ± 0.014
0.020 ± 0.005
360
224.3 ± 13.14
1.91 ± 0.13
7.40 ± 0.60∗∗
1.34 ± 0.13
0.71 ± 0.08
0.33 ± 0.06
0.43 ± 0.06∗∗
0.50 ± 0.20∗∗
0.141 ± 0.025
0.093 ± 0.013
0.020 ± 0.005
720
213.4 ± 11.75∗∗
1.88 ± 0.07
9.21 ± 0.54∗∗
1.36 ± 0.10
0.69 ± 0.06
0.30 ± 0.03
0.36 ± 0.05∗∗
0.46 ± 0.12∗∗
0.140 ± 0.037
0.100 ± 0.012
0.023 ± 0.009
Historical range♮
206–285
1.75–2.18
5.30–7.97
1.23–2.00
0.66–0.96
0.24–0.54
0.33–0.75
0.40–2.07
0.098–0.208
0.063–0.113
0.012–0.029
Female (recovery group, = 5 each)
Control
248.4 ± 8.73
2.04 ± 0.05
6.63 ± 0.45
1.57 ± 0.11
0.74 ± 0.05
0.38 ± 0.05
0.49 ± 0.04
0.72 ± 0.10
0.139 ± 0.030
0.071 ± 0.009
0.022 ± 0.002
720
217.2 ± 9.58∗∗
1.92 ± 0.03∗∗
5.86 ± 0.21∗∗
1.37 ± 0.07∗∗
0.67 ± 0.04
0.33 ± 0.03
0.43 ± 0.04
0.48 ± 0.10∗∗
0.130 ± 0.031
0.075 ± 0.005
0.024 ± 0.002
Data represent the mean values and the standard deviation. and ∗∗ P < 0.01, statistical significances were determined with Duncan's multiple range test or with Mann–Whitney test versus control. ♮: minimum and maximum levels reported as the range of historical control values.
