An Assessment of the Genotoxicity and Subchronic Toxicity of a Supercritical Fluid Extract of the Aerial Parts of Hemp
Table 10
Summary of selected† hematological findings in the 90-day repeated oral toxicity study.
Group
NEU
LYM
MONO
EOS
RBC
HGB
HCT
MCV
MCH
MCHC
PLT
RET
APTT
(mg/kg bw/day)
%
%
%
%
×1012/L
g/L
L/L
fL
pg
g/L
×109/L
%
sec
Male (main groups, = 10 each)
Control
14.21 ± 2.44
82.56 ± 2.64
2.20 ± 0.40
0.99 ± 0.31
9.46 ± 0.28
167.60 ± 4.99
0.46 ± 0.01
49.06 ± 1.52
17.71 ± 0.43
361.00 ± 4.24
787.30 ± 90.20
3.01 ± 0.44
21.73 ± 3.64
100
19.25 ± 3.80∗∗
77.81 ± 4.17
2.13 ± 0.38
0.77 ± 0.29
9.49 ± 0.54
166.70 ± 6.73
0.46 ± 0.02
48.97 ± 1.92
17.59 ± 0.59
359.50 ± 5.15
804.00 ± 93.50
3.26 ± 0.33
22.67 ± 1.59
360
16.40 ± 4.50
81.30 ± 4.88
1.85 ± 0.46
0.44 ± 0.20∗∗
9.72 ± 0.47
168.80 ± 6.99
0.47 ± 0.02
47.96 ± 1.47
17.39 ± 0.45
362.30 ± 5.19
848.80 ± 79.82
3.03 ± 0.27
25.48 ± 4.32
720
18.03 ± 3.98
79.70 ± 4.19
1.84 ± 0.57
0.42 ± 0.25∗∗
9.41 ± 0.55
158.30 ± 6.85∗∗
0.44 ± 0.02∗∗
46.75 ± 2.25
16.86 ± 0.59∗∗
360.60 ± 6.17
845.10 ± 58.72
3.17 ± 0.35
22.45 ± 2.56
Historical range♮
9.7–38.7
56.5–87.8
1.8–5.1
0.3–9.0
7.52–10.21
141–180
0.404–0.489
45.4–53.7
16.6–18.8
349–379
595–957
2.05–4.65
15.2–31.8
Male (recovery groups, = 5 each)
Control
15.42 ± 4.77
80.12 ± 5.80
3.06 ± 0.82
1.34 ± 0.49
9.90 ± 0.56
171.60 ± 5.86
0.47 ± 0.02
47.34 ± 2.40
17.34 ± 0.77
367.00 ± 4.64
831.00 ± 91.48
3.39 ± 0.20
19.80 ± 3.67
720
20.94 ± 6.68
75.74 ± 6.45
2.20 ± 0.66
1.08 ± 0.33
9.66 ± 0.44
167.00 ± 7.91
0.46 ± 0.03
47.56 ± 2.04
17.28 ± 0.38
363.80 ± 8.47
758.00 ± 125.59
3.62 ± 0.28
21.02 ± 1.82
Female (main groups, = 10 each)
Control
11.49 ± 4.30
85.13 ± 4.83
2.06 ± 0.59
1.30 ± 0.54
8.49 ± 0.39
159.80 ± 2.86
0.45 ± 0.01
52.88 ± 2.90
18.87 ± 0.83
356.90 ± 5.47
753.80 ± 94.29
3.95 ± 0.68
21.01 ± 2.26
100
18.80 ± 5.14∗∗
78.45 ± 5.32∗∗
1.93 ± 0.50
0.82 ± 0.53
8.77 ± 0.25
159.70 ± 5.01
0.45 ± 0.02
50.84 ± 1.75
18.20 ± 0.46
358.30 ± 5.52
788.60 ± 63.52
3.70 ± 0.69
23.73 ± 4.91
360
13.99 ± 2.85
83.81 ± 3.08
1.60 ± 0.44
0.60 ± 0.22∗∗
8.89 ± 0.37
156.90 ± 5.32
0.44 ± 0.01
49.54 ± 2.02∗∗
17.66 ± 0.67∗∗
356.50 ± 3.31
835.10 ± 73.32
3.35 ± 0.45
21.49 ± 2.74
720
14.06 ± 3.28
83.65 ± 3.53
1.47 ± 0.37
0.80 ± 0.40
8.99 ± 0.25∗∗
155.30 ± 4.72
0.43 ± 0.01
48.26 ± 0.75∗∗
17.28 ± 0.29∗∗
358.00 ± 5.03
757.50 ± 63.89
3.06 ± 0.47∗∗
20.87 ± 3.40
Historical range♮
5.8–33.3
63.4–91.1
1.1–3.9
0.4–2.1
7.71–9.17
148–168
0.415–0.467
47.0–60.1
17.2–20.8
346–372
549–1103
2.77–5.63
17.4–27.9
Female (recovery groups, = 5 each)
Control
19.62 ± 6.96
77.26 ± 6.93
1.84 ± 0.38
1.22 ± 0.35
8.88 ± 0.32
161.80 ± 5.36
0.45 ± 0.01
50.74 ± 0.94
18.22 ± 0.33
359.60 ± 3.51
817.20 ± 97.76
3.79 ± 0.60
23.94 ± 4.60
720
21.38 ± 5.64
75.60 ± 5.43
1.60 ± 0.74
1.42 ± 0.48
8.99 ± 0.30
160.60 ± 7.23
0.44 ± 0.02
49.30 ± 2.49
17.88 ± 0.81
362.80 ± 2.39
900.00 ± 129.28
3.73 ± 0.62
21.50 ± 5.65
Data represent the mean values and the standard deviation. : only parameters with statistically significant findings are shown in table. and ∗∗P < 0.01, statistical significances were determined with Duncan's multiple range test or with Mann–Whitney test versus control. : minimum and maximum levels reported as the range of historical control values.