An Assessment of the Genotoxicity and Subchronic Toxicity of a Supercritical Fluid Extract of the Aerial Parts of Hemp

Table 1

Summary table of the results of the initial mutation test.

Initial mutation test (plate incorporation test)
Concentrations (g/plate)	Salmonella typhimurium tester strains																Escherichia coli
	TA 98				TA 100				TA 1535				TA 1537				WP2uvrA
	-S9		+S9		-S9		+S9		-S9		+S9		-S9		+S9		-S9		+S9
Mean values of revertants per plate and mutation rate (MR)	Mean	MR	Mean	MR	Mean	MR	Mean	MR	Mean	MR	Mean	MR	Mean	MR	Mean	MR	Mean	MR	Mean	MR
Untreated control	13.7	0.89	19.3	1.09	84.0	1.02	105.0	1.12	8.7	1.18	11.7	1.03	12.7	0.93	11.3	0.89	13.3	0.95	21.7	1.16
DMSO control	15.3	1.00	17.7	1.00	82.0	1.00	93.7	1.00	7.3	1.00	11.3	1.00	13.7	1.00	12.7	1.00	14.0	1.00	18.7	1.00
Ultrapure Water control	–	–	–	–	83.3	1.00	–	–	8.3	1.00	–	–	–	–	–	–	14.0	1.00	–	–
5000	11.0	0.72	14.0	0.79	54.0	0.66	78.0	0.83	3.7	0.50	4.7	0.41	4.7	0.34	2.0	0.16	13.0	0.93	18.0	0.96
1600	13.3	0.87	18.0	1.02	56.0	0.68	70.3	0.75	9.3	1.27	8.0	0.71	12.7	0.93	9.7	0.76	10.7	0.76	18.0	0.96
500	12.3	0.80	22.0	1.25	60.7	0.74	86.3	0.92	8.7	1.18	10.0	0.88	10.0	0.73	12.0	0.95	11.3	0.81	19.3	1.04
160	11.0	0.72	23.0	1.30	89.3	1.09	92.3	0.99	8.0	1.09	11.3	1.00	11.7	0.85	12.7	1.00	14.3	1.02	19.7	1.05
50	15.3	1.00	24.0	1.36	98.3	1.20	87.0	0.93	7.3	1.00	9.7	0.85	12.0	0.88	13.7	1.08	11.0	0.79	16.7	0.89
16	15.3	1.00	20.0	1.13	86.0	1.05	91.7	0.98	9.0	1.23	9.0	0.79	12.0	0.88	12.7	1.00	14.0	1.00	17.7	0.95
5	13.3	0.87	17.0	0.96	84.3	1.03	101.0	1.08	9.0	1.23	12.0	1.06	13.3	0.98	12.3	0.97	14.0	1.00	18.3	0.98
NPD (4)	208.0	13.57	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
SAZ (2)	–	–	–	–	1008.0	12.10	–	–	1066.7	128.00	–	–	–	–	–	–	–	–	–	–
9AA (50)	–	–	–	–	–	–	–	–	–	–	–	–	574.0	42.00	–	–	–	–	–	–
MMS (2 μL)	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	422.7	30.19	–	–
2AA (2)	–	–	1469.3	83.17	–	–	2506.7	26.76	–	–	105.0	9.26	–	–	136.7	10.79	–	–	–	–
2AA (50)	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	209.0	11.20

DMSO was used as the vehicle for the test article and positive control substances: NPD, 9AA, and 2AA. Ultrapure water was used as the vehicle for SAZ and MMS. The mutation rate of the test item and the untreated control is given referring to the DMSO.