Research Article
TGF-β Signaling Cooperates with AT Motif-Binding Factor-1 for Repression of the α-Fetoprotein Promoter
Table 1
PCR primers to amplify human cDNAs.
| Human cDNA | | Sequence | | AT | Cycle |
| AFP | (+) | ATCCAGGAGAGCCAAGCATT | 434 | 58°C | 20 | (−) | TTCATCGTTTGCAGCGCTAC |
| ATBF1 | (+) | TGGCATCAAGTACAGCGCTC | 392 | 53°C | 35 | (−) | GAACAGTTGTGCTGGGCAGA |
| TMEPAI | (+) | GATCATCATCATCGTGGTGG | 455 | 60°C | 35 | (−) | CACTGTCGAAGATGGTTCTG |
| -actin | (+) | CACCCACACTGTGCCCATCTACGA | 410 | 63°C | 22 | (−) | TGGCGTACAGGTCTTTGCGGATGT |
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: length of PCR fragment; AT: annealing temperature.
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