A Novel Interaction between Pyk2 and MAP4K4 Is Integrated with Glioma Cell Migration

<table>Increased expression of MAP4K4 stimulates migration but is blocked by knockdown of Pyk2 expression. (a) Wild-type SF767 glioma cells (CTL) or SF767 cells stably transduced with a shRNA targeting Pyk2 (Pyk2i) were transfected with vector or HA-epitope-tagged MAP4K4. Whole cell lysates were blotted with the indicated antibodies. Lysates were blotted with actin as a loading control. (b) SF767 control cells or SF767 cells with shRNA mediated knockdown of Pyk2 (Pyk2i) were transfected with MAP4K4, and the migration rate on 10 <i>μ</i>g/mL laminin was assessed over 24 hr using a radial migration assay. <sup>†</sup><svg height="11.375" id="M20" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 75.025002 11.375" width="75.025002" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg> relative to control.</table>

Journal of Signal Transduction

fig6

Figure 6

Figure 6: A Novel Interaction between Pyk2 and MAP4K4 Is Integrated with Glioma Cell Migration 