A Novel Interaction between Pyk2 and MAP4K4 Is Integrated with Glioma Cell Migration
Figure 5
Knockdown of MAP4K4 expression inhibits glioma cell migration and invasion and blocks Pyk2 mediated stimulation of migration. (a) SF767 cells were transiently transfected with shRNA 1M4K4i and shRNA 2M4K4i or cotransfected with shRNAs 1M4K41i and 2M4K4i. Cell lysates were blotted with indicated antibodies. (b) SF767 cells were stably transduced with lentivirus expressing 2M4K4i shRNA. Stably transduced cells (solid histogram) were enriched by flow cytometry. Open histogram: control SF767 parental cells. Inset: whole cell lysates of control SF767 cells or SF767 cells stably transduced with MAP4K4 shRNA (MAP4K4i) were immunoblotted with the indicated antibodies. (c) The migration rate of control SF767 cells, SF767 cells stably transduced with a shRNA targeting MAP4K4 (MAP4K4i), and MAP4K4i cells transfected with Pyk2 was assayed with a radial migration assay on 10 μg/mL laminin substrate. * compared to control. (d) Transwell invasion assay of control SF767 cells, SF767 cells stably transduced with a shRNA targeting MAP4K4 (MAP4K4i), or SF767 cells stably transduced with a shRNA targeting Pyk2 (Pyki). * compared to control.
