Upregulation of Voltage-Gated Calcium Channel Ca_v1.3 in Bovine Somatotropes Treated with Ghrelin

Figure 8

Expression of L-type Ca²⁺ channel 𝛼1 subunits mRNA in bovine somatotropes. (a) Electrophoresis of L-type Ca²⁺ channel a 1.2 and 1.3 subunits mRNA, bGH, and 𝛽-actin (B-Ac). (b) Summary of three independent experiments; the principal subunit expressed is 1.3. (c) Electrophoresis of somatotropes cells treated by GHRP-6 100 nM for 48 h. (d) Summary of three independent experiments; GHRP-6 increases the mRNA for HVA calcium channels L-type 1.3 and the mRNA for bGH.

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