Upregulation of Voltage-Gated Calcium Channel Ca_v1.3 in Bovine Somatotropes Treated with Ghrelin

Figure 5

Treatment with Ghrelin enhances Ca²⁺ channel activity in bovine somatotropes. (a) Voltage-activated Ba²⁺ currents (through Ca²⁺ channels) evoked by depolarizing test pulses from a Vh of −80 mV with a 10 mV interval (as indicated in the lower panel) in bovine GH cells kept in culture in the absence (control) or presence of Ghrelin (10 nM) for 48 h. (b) Average current-voltage (𝐼-𝑉) relationships obtained from control (⬤,𝑛=5) and treated cells (⚪, 𝑛=6) measured at the peak of each current trace. (c) Average 𝐼-𝑉 curves obtained as in (a); current values were obtained at the end of the command pulse (□: Control; ■: Ghrelin).