Upregulation of Voltage-Gated Calcium Channel Ca_v1.3 in Bovine Somatotropes Treated with Ghrelin

Figure 2

Ghrelin and GHRP-6 enhance secretory activity in bovine somatotropes. (a) Ghrelin (10 nM) significantly stimulates GH secretion in bovine GH cells. (b) GH-stimulated secretion by GHS is mediate through GHS-R activation because the treatment with the antagonist of GHS-R (DL3-GHRP-6) blocked GH secretion; Ghrelin (10 nM), GHRP-6 (100 nM), GHRP-6 (100 nM) + DL3-GHRP-6 (10 μM), Ghrelin (10 nM) + DL3-GHRP-6 (10 μM). (c) Dose response curve of GH secretion stimulated by GHRP-6 (48 h). (d) Time course of GH secretion stimulated by GHRP-6 (100 nM).

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