An Fc Gamma Receptor-Mediated Upregulation of the Production of Interleukin 10 by Intravenous Immunoglobulin in Bone-Marrow-Derived Mouse Dendritic Cells Stimulated with Lipopolysaccharide In Vitro
Figure 6
Anti-FcγRI antibody affects the cytokine productions in the presence of IVIG in BMDC stimulated with LPS. The anti-mouse FcγRI antibody and a matched control antibody were added to the cells in the presence or absence of IVIG (10 mg/mL) and cells were stimulated with LPS (500 ng/mL) for 18 h. Productions of cytokines in the culture supernatant were measured using respective ELISA kits and results were expressed as mean ± SEM . , significantly different from the medium alone (without LPS stimulation, Student’s t-test); , , significantly different from the Control (LPS stimulation without IVIG, Dunnett’s multiple comparison test). ND, Cytokine concentrations were under the lower limit values of the standard ELISA curves. Three independent experiments were conducted and representative results were shown. (a) IL-10; (b) IL-12p70.
