An Fc Gamma Receptor-Mediated Upregulation of the Production of Interleukin 10 by Intravenous Immunoglobulin in Bone-Marrow-Derived Mouse Dendritic Cells Stimulated with Lipopolysaccharide In Vitro
Figure 1
IVIG increased IL-10 production whereas decreased IL-12p70 production in BMDC stimulated with LPS. Cells were stimulated with LPS (1 μg/mL) in the presence of IVIG or human pooled plasma for 18 h. Cytokine concentrations in the culture medium were determined using respective ELISA kits. (a) Production of IL-10; (b) production of IL-12p70; (c) production of IL-10 in the presence of human pooled plasma. Results were expressed as mean ± SEM . , significantly different from the medium alone (without LPS stimulation, Student’s t-test); , , significantly different from the Control (LPS stimulation without IVIG, Dunnett’s multiple comparison test); , significantly different from the Control, Student’s t-test; ND, IL-10 and IL-12p70 concentrations were under the lower limit values of the standard ELISA curves. At least three independent experiments were conducted and representative results were shown.
