Prolactin and Dexamethasone Regulate Second Messenger-Stimulated Cl⁻ Secretion in Mammary Epithelia

Figure 6

Effect of cAMP cocktail on iodide efflux in prolactin and dexamethasone treated, vector- (a) or PRLR-S-transfected (b) HC-11 cells. Vector transfected HC-11 cells (vtc, (a)) and PRLR-S-transfected cells (rs, (b)) were treated with 1 μg/mL prolactin and dexamethasone (P-D) for 24 h prior to the iodide efflux assays. Iodide efflux assays were performed in the absence or presence of the cAMP cocktail (100 μM cAMP, 10 μM forskolin, 100 μM IBMX). Data represent mean ± SEM relative to value at starting point. 𝑛≥3. *𝑃<0.05 versus non-cAMP treated groups.

(a)

(b)