Prolactin and Dexamethasone Regulate Second Messenger-Stimulated Cl⁻ Secretion in Mammary Epithelia

Figure 5

((a1) and (a2)) mRNA expression of CFTR (234 bp) (a1), and ClC1 (179 bp), ClC2 (221 bp) (a2) in HC-11 cells. Representative agarose gel image shows ethidium bromide-stained PCR products. (b1)–(b3) effect of prolactin and/or dexamethasone treatment on CFTR (b1), NKCC1 (b2), and ClC2 (b3) mRNA expression in HC-11 cells. HC-11 cells were treated with or without 1 μg/mL prolactin (P) and/or dexamethasone (D) for 24 h. Total RNA was extracted, and realtime PCR was performed. Ribosomal protein L19 mRNA was used as internal control. C = control. Data represent mean ± SEM relative to control, 𝑛=4 for CFTR and NKCC1, and 𝑛=3 for ClC2. *𝑃<0.05  versus control.