The Dynamical Systems Properties of the HOG Signaling Cascade
Figure 4
Different microfluidics techniques to control the chemical environment of single yeast cells while imaging them through microscopy. (a) Microfluidic system as described in Hersen et al. [11]. Yeast cells are fixed in the channel by the lectin protein Concanavalin A. One inlet is filled with an iso-osmotic media (blue) and the other with a hyperosmotic media (orange). By tightly controlling the pressure in each inlet, it is possible to create a periodic shock on the cells. (b) Optical tweezers system (red) as described by Eriksson et al. permits to control the cells position in the channel with two fluids flowing side by side [12]. (c) The system developed by Charvin et al. uses a dialysis membrane (green) to trap cells on top of a soft PDMS slice [13]. (d) Multilayer microfluidic device [14]. The top layer (green) is used to capture cells. By controlling the pressure inside this channel, cells can be optimally trapped while subjected to periodic shocks. The bottom layer is used to culture cells.
