Role of RNA-Binding Proteins in MAPK Signal Transduction Pathway
Figure 1
Molecular genetic approach to identify regulators of MAPK signaling using fission yeast model system. Upper panel: Calcineurin phosphatase and Pmk1 MAPK pathway play antagonistic roles in Cl− homeostasis of fission yeast. Molecular genetic approach utilizing this genetic interaction identified regulators of Pmk1 MAPK. Lower panel: Calcineurin deletion cells (CN KO) failed to grow in the presence of 0.15 M MgCl2. Overexpression of multicopy suppressor genes, including pmp1+ (MAPK phosphatase), pek1+ (MAPK kinase), and rnc1+ (KH-type RNA-binding protein) suppressed the Cl− sensitivity of the calcineurin deletion cells.
