Rapid Screening and Estimation of Binding Constants for Interactions of Fe³⁺ with Two Metalloproteins, Apotransferrin and Transferrin, Using Affinity Mode of Capillary Electrophoresis

Table 3

Statistical components of four plotting methods used for the calculation of binding constant (K).

Plotting methods	Fitting method	K	Reference
Nonlinear regression		Slope	[22, 35]
X-reciprocal	versus	Slope	[22, 35]
Y-reciprocal	versus c (L)		[22, 35]
Double-reciprocal	versus		[22, 35]

K: binding constant; c (L): micromolar concentration of ligand (metal ion); Rf and Ri: mobility ratios of the protein before and after interaction with the ligand in the capillary tube, respectively; Rc: mobility ratio of analyte protein at saturated ligand concentration.