Rapid Screening and Estimation of Binding Constants for Interactions of Fe³⁺ with Two Metalloproteins, Apotransferrin and Transferrin, Using Affinity Mode of Capillary Electrophoresis

Table 2

Average ΔR/Rf values and their confidence intervals (cnf) for transferrin- and apotransferrin-iron (III) interactions at different iron (III) ion concentrations.

Iron (III) ion concentrations (μmol/L)	Transferrin-iron (III) ion interaction	Apotransferrin-iron (III) ion interaction
	ΔR/Rf ± cnf	ΔR/Rf ± cnf
5	−0.0007 ± 0.0128	−0.0042 ± 0.0019
10	0.0012 ± 0.0085	0.0021 ± 0.0062
25	−0.0007 ± 0.0037	−0.0008 ± 0.0035
50	0.0114 ± 0.0141	0.0035 ± 0.0096
100	−0.0201 ± 0.0090	−0.0254 ± 0.0047
150	−0.0075 ± 0.0101	0.0070 ± 0.0080
250	−0.0012 ± 0.0169	−0.0205 ± 0.0020

Average values; n = 6.