Automation of the Peak Fitting Method in Bone FTIR Microspectroscopy Spectrum Analysis: Human and Mice Bone Study

Table 2

Differences of the manual peak fitting analysis intraoperator, interoperator, maximum absolute error, and variation of results using the automatic method for each parameter, based on the analysis of 5 bone spectra. Operator 1 is an experienced operator, and operator 2 is an inexperienced operator to whom has been given the instructions to analyse the spectra.

	Crystallinity Index	Mineral Maturity	Collagen Maturity
RMS of coefficient of variation (operator 1) (%)	0.7	1.4	0.7
RMS of coefficient of variation (operator 2) (%)	1.7	0.4	1.0
RMS of coefficient of variation (interoperator) (%)	2.0	1.2	0.7
Maximum absolute error	0.0013 cm	0.07	0.11
Variation of results using automatic method	No variation	No variation	No variation

RMS = root mean square.