Review Article

Patched Knockout Mouse Models of Basal Cell Carcinoma

Figure 1

Features of BCC in 𝑃 𝑡 𝑐 𝑓 𝑙 𝑜 𝑥 / 𝑓 𝑙 𝑜 𝑥 𝐸 𝑅 𝑇 2 + / and 𝑃 𝑡 𝑐 𝑓 𝑙 𝑜 𝑥 / 𝑓 𝑙 𝑜 𝑥 𝐾 5 - 𝐶 𝑟 𝑒 - 𝐸 𝑅 𝑇 mice. 𝑃 𝑡 𝑐 𝑓 𝑙 𝑜 𝑥 / 𝑓 𝑙 𝑜 𝑥 animals were bred with the mouse lines ERT2 or 𝐾 5 - 𝐶 𝑟 𝑒 - 𝐸 𝑅 𝑇 . The respective Ptch flox/+ 𝐸 𝑅 𝑇 2 + / and 𝑃 𝑡 𝑐 𝑓 𝑙 𝑜 𝑥 / + 𝐾 5 - 𝐶 𝑟 𝑒 - 𝐸 𝑅 𝑇 mice were backcrossed to 𝑃 𝑡 𝑐 𝑓 𝑙 𝑜 𝑥 / 𝑓 𝑙 𝑜 𝑥 mice to obtain 𝑃 𝑡 𝑐 𝑓 𝑙 𝑜 𝑥 / 𝑓 𝑙 𝑜 𝑥 𝐸 𝑅 𝑇 2 + / and 𝑃 𝑡 𝑐 𝑓 𝑙 𝑜 𝑥 / 𝑓 𝑙 𝑜 𝑥 𝐾 5 - 𝐶 𝑟 𝑒 - 𝐸 𝑅 𝑇 mice. ERT2 or 𝐾 5 - 𝐶 𝑟 𝑒 - 𝐸 𝑅 𝑇 was activated by one intramuscular (i.m.) injection of 100 μg tamoxifen as described recently [20, 85], or by intraperitoneal injections of 1 mg tamoxifen (10 μg/μL in sterile ethanol/sun flower oil 1 : 25) for 5 consecutive days (see [88]), respectively. Genotyping of the 𝑃 𝑡 𝑐 𝑓 𝑙 𝑜 𝑥 , 𝑃 𝑡 𝑐 𝑑 𝑒 𝑙 , ERT2, and 𝐾 5 - 𝐶 𝑟 𝑒 - 𝐸 𝑅 𝑇 alleles was performed as described recently [20, 84, 85]. All mice used in the study were handled in accordance with the German animal protection law. (a) shows the appearance and histology of skin from control and tamoxifen-treated 𝑃 𝑡 𝑐 𝑓 𝑙 𝑜 𝑥 / 𝑓 𝑙 𝑜 𝑥 𝐸 𝑅 𝑇 2 + / mice and (b) shows that of control and tamoxifen-treated 𝑃 𝑡 𝑐 𝑓 𝑙 𝑜 𝑥 / 𝑓 𝑙 𝑜 𝑥 𝐾 5 - 𝐶 𝑟 𝑒 - 𝐸 𝑅 𝑇 mice.
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(a)
907543.fig.001b
(b)