In Vitro Filaricidal Properties of Aqueous Extracts of Combretum nigricans (Combretaceae) on Onchocerca ochengi (Onchocercidae)

Figure 2

Macro- and microfilaricidal activities of various aqueous extracts of Combretum nigricans on Onchocerca ochengi in comparison to that of flubendazole and ivermectin and dose-response curves. Adult worms (6/well) were incubated in 24-well plates in the RPMI-1640 medium at 37°C in a humidified atmosphere in the presence of flubendazole (Flu.) at 0.3 mg/mL or the aqueous extracts of leaves, barks, and roots at 1 mg/mL for 24 h, and the dose-response study was conducted to record the inhibition concentration 50 (IC₅₀) values. Microfilariae (17/well) were incubated with the plant extracts or ivermectin (IVM) at 0.5 mg/mL for the screening, and the IC₅₀ was determined using motility inhibition tests. Data were expressed in . , , , and when compared with the flubendazole or the ivermectin-treated group.

(a)

(b)

(c)

(d)