DNA-Detection Based Diagnostics for Taenia solium Cysticercosis in Porcine

Table 5

Comparison between established PCR and most common isothermal amplification methods.

	PCR	LAMP	RPA
Sample processing prior to amplification	Nucleic acid extraction required	Amplification from crude samples possible	Amplification from crude samples possible [22]
Simplicity of operation	Complex	Comparatively easier to setup than PCR	Relatively easier to setup than PCR
Cost	$6–7.7 [70]	$0.71–2	$4.3−4.7 [64, 69]
Stability of reagent	Cold chain required for enzymes	Cold chain required for enzymes	Reagents available as dry pellet [17]
		Reagents available as dry pellet [52, 53]
Skills required	High	Moderate	Moderate
Reaction time	2 h [14]	60−90 mins [13, 21]	15−30 mins [22]
Amplification temperature	Varying [32, 33]	65°C [43]	25−42°C [17, 22]
Sensitivity	High	High	High
Specificity	High	High	High
Limit of detection	1 ng [25, 70]	5 eggs/g of feces [13]	Unknown
Taenia solium identification	Yes [14, 26, 27]	Yes [12, 13, 42]	Possible
Field tested	No	Yes	No
Product developer	Qiagen, Hilden, German [70]	New England Biolabs, Hitchin, UK	TwistDx, Cambridge, UK [20]