Research Article

Applicability of the Filter Paper Technique for Detection of Antifilarial I g G 4 Antibodies Using the Bm14 Filariasis CELISA

Table 2

Cross-tabulation results for the Filariasis CELISA comparing reactivity from paired filter paper samples following 10-month storage at -20°C. Following storage of filter paper samples for 10 months, the reactivity of the samples reduced (P<.001). Sixty-seven samples became nonreactive following storage dropping the sensitivity to 34% (95%-CI 25–44) and the negative predictive value (NPV) to 60% (95%-CI 52–67). Specificity (100%) and the positive predictive value (PPV) (100%) remained unchanged following storage (95%-CI 96–100 and 95%-CI 90–100 resp.).

Initial Result

NegativePositiveTotal
Following storageNegative9967166
NPV60% (99/166)
Specificity100% (99/99)
Positive03434
PPV100% (34/34)
Sensitivity34% (34/101)
Total99101200