Applicability of the Filter Paper Technique for Detection of AntifilarialIgG₄ Antibodies Using the Bm14 Filariasis CELISA

Table 1

Cross-tabulation results for the Filariasis CELISA comparing paired plasma and filter paper samples. The gold standard was considered to be the result derived from the plasma sample (columns). ELISA specifications for a filter paper eluate was found to be a sensitivity of 92% (95%-CI = 75–99), specificity of 77% (95%-CI = 65–86), positive predictive value (PPV) of 60% (95%-CI = 43–75), and negative predictive value (NPV) of 96% (95%-CI = 87–100).

		Plasma
		Negative	Positive	Total
Filter paper	Negative	52	2	54
	NPV	96% (52/54)
	Specificity	77% (52/68)
	Positive	16	24	40
	PPV		60% (24/40)
	Sensitivity		92% (24/26)
	Total	68	26	94